Formazione

 

1/04/2015 – 30/09/2018

Doctor of Philosophy (Ph.D.) in Agricultural Science, Gifu University, Japan

1/04/2013 – 30/03/2015

Master of Science (M.Sc.) in Applied Biological Science, Gifu University, Japan

1/09/2005 – 31/01/2010

Bachelors of Science (B.Sc.) in Plant Protection, University of Jordan, Jordan

Carriera accademica ed attività didattica
1/08/2023 – currentPostdoctoral researcher, University of Trento, Italy
1/08/2021 – 31/07/2023 Postdoctoral researcher, European Union’s
Horizon 2020 project “FreezingBioprotector”
University of Trento, Italy
1/08/2020 – 31/07/2021Postdoctoral researcher, University of Trento, Italy
1/10/2019 – 31/07/2020Postdoctoral researcher, Ibaraki University, Japan
1/10/2019 – 1/12/2019Teaching assistant, Ibaraki University, Japan
20/10/2018 – 30/09/2019Postdoctoral researcher, Gifu University, Japan
1/04/2018 – 30/09/2018Research assistant, Gifu University, Japan
1/04/2013 – 30/09/2013Teaching assistant, Gifu University, Japan
1/04/2012 – 30/03/2013Researcher, Gifu University, Japan
Interessi di ricerca
  • Ecology and evolution of plant-associated and environmental microbiomes in natural and managed environments.
  • Plant-microbiome-mobilome interactions and (co)evolution under stress conditions.
  • Genetic and biochemical features of plant-microbiome-mobilome interactions.
Attività di ricerca

My research career as a plant pathologist started as a Master’s student where I focused on evaluating the suppressive effect of Allium intercropping on tomato bacterial wilt, and on the bacterial community structure of Alliums and tomato. Afterward, as a Ph.D. student, I utilized the bacterial community data to develop novel biocontrol agents against tomato bacterial wilt, which was the main research area of my dissertation. In this study, I succeeded in developing a practical biocontrol strategy against tomato bacterial wilt.

As a postdoctoral researcher (I), I have been investigating the competition for nutrients as one of the mechanisms employed by the newly discovered microbial biocontrol agents (MBCAs; Mitsuaria and non-pathogenic Ralstonia) against the tomato bacterial wilt pathogen (Ralstonia pseudosolanacearum), genome analysis of these MBCAs and the pathogen, transcriptome analysis of tomato plant following MBCAs treatment and pathogen challenge inoculation.

As a postdoctoral researcher (II), I have been studying the various interactions between dark septate fungal root endophytes - host plants (ornamentals and fruit crops) - pathogens (oomycete and fungi). In particular, I succeeded in developing a practical biocontrol strategy using a new DSE against Pythium root rot on eustoma plant for hydroponic culture system.

As a Marie-Curie fellow, I was awarded “MSCA individual fellowship” from the European Commission within Horizon 2020 framework to continue my research project on
“Understanding the role of microbiome interactions in the cold stress tolerance of plants”. Climate change-associated extreme weather events such as early spring frosts have increased in frequency and intensity over the past decades. Cold stress is one of the main limiting factors for fruit crop production worldwide. Plants growing in cold regions are hypothesized to survive cold stress thanks to symbiosis with endophytic microorganisms. However, the structure and function of endophytic microbial communities associated with such plants are poorly understood. Among the major findings is that (i) plant tissue, collection site and host plant were the main factors affecting richness, diversity and taxonomic structure of endophytic bacterial communities in flowers, leaves and roots of alpine Rosaceae plants and (ii) psychrotolerant bacterial endophytes belonging to the core taxa of Duganella, Erwinia, Pseudomonas and Rhizobium genera, mitigated freezing stress in strawberry plants, demonstrating the beneficial role of endophytic bacterial communities (Marian et al., 2023; preprint). Several research lines emerged from my Marie-Curie project. This includes the identification of several bacterial isolates, that provided efficient freezing mitigation, belonging to Erwinia, Duganella, Pseudomonas and Rhizobium. I am currently focusing on better understanding the mechanisms of bacterial-mediated enhanced freezing stress tolerance on both strawberry and apple plants. In particular, the upregulation of important plant genes and pathways, alleviation of reactive oxygen species (ROS) and membrane lipid peroxidation, enhancement of ROS scavenging activities and improved osmolyte substances production. Furthermore, I am focusing on the diversity and structure of phage communities and their role in plant-bacteria interactions in Alpine areas.

Appartenenza a società e comitati scientifici
  • The Phytopathological Society of Japan, 2016 –
  • Japanese Society of Soil Microbiology, 2017 –
  • International Organization for Biological and Integrated Control/West Palaearctic Regional Section, 2018 –
  • Society for Applied Microbiology, 2021  –
  • International Society for Microbial Ecology (ISME), 2022 –
Premi e riconoscimenti
2021Award for the Most Valuable Paper from Microbes and Environments Journal. Paper entitled “Potential use of L-arabinose for the control of tomato bacterial wilt”
2020Award for Paper of Excellence from the The Phytopathological Society of Japan. Paper entitled “Enhanced biocontrol effect using the combined application of Mitsuaria sp. TWR114 and non-pathogenic Ralstonia sp. TCR112 for controlling tomato bacterial wilt”
2020Seal of Excellence: MSCA-IF High Quality Project, awarded by European Commission for the proposal submitted under the Horizon 2020 Marie Skłodowska-Curie actions call H2020-MSCA-IF-2019.
2018Gold award for best student oral presentation. Presentation title “Novel isolates of Ralstonia and Mitsuaria species as biocontrol agents for controlling tomato bacterial wilt”, IOBC-WPRS (International Organization for Biological and Integrated Control-West Palaearctic Regional Section) XV Meeting of the Working Group “Biological and integrated control of plant pathogens”, Lleida, Spain
04/2012 – 03/ 2018Japanese Government Scholarship from The Ministry of Education, Culture, Sports, Science, and Technology (Monbugakusho: MEXT)
11/2009 – 01/2010Hashemite Kingdom of Jordan Government Scholarship from The Ministry of Higher Education and Scientific Research
Convegni e conferenze
  • Marian M, Milanese I, Antonielli L, Perazzolli M. (2022). Taxonomic and functional analysis of the endophytic microbiome associated with wild cold-adapted Rosaceae plants. Poster presentation at the Copenhagen Bioscience Conference: Plant-microbe Interactions, Copenhagen, Denmark (Poster).
  • Marian M, Antonielli L, Milanese I, Perazzolli M. (2022). Exploring the functional potential of plant-associated microbiomes in alpine region to enhance cold stress tolerance in plants. Oral communication and Abstract. 9th International Conference on Polar and Alpine Microbiology. 9-14 October, Potsdam, Germany (Oral).
  • Marian M, Milanese I, Antonielli L, Pertot I, Perazzolli M. (2022). Deciphering the anthosphere and endosphere microbiome of wild Rosaceae plants for cold-adapted plant growth promoting bacteria. Poster presentation at the INTERNATIONAL SYMPOSIUM MICROBE-ASSISTED CROP PRODUCTION - OPPORTUNITIES, CHALLENGES & NEEDS (miCROPe), Vienna, Austria (Poster). 
  • Marian M., Morita A., Koyama H., Suga H., and Shimizu M. (2019). Synergistic effect of Mitsuaria sp. TWR114 and Ralstonia sp. TCR112 on enhanced biocontrol of tomato bacterial wilt. 5th Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation. Sendai, Japan (Poster).
  • Wu R., Marian M., Koyama H., Suga H., Kageyama K., and Shimizu M., Characterization of competition for nutrients in the biocontrol of tomato bacterial wilt by Mitsuaria sp. TWR114 and Ralstonia sp. TCR112. Annual Meeting of the Japanese Society of Soil Microbiology. Hokkaido, Japan (Poster).
  • Wu R., Marian M., Koyama H., Suga H., Kageyama K., and Shimizu M (2019). Role of nutrient competition in biocontrol of tomato bacterial wilt by Mitsuaria sp. TWR114 and Ralstonia sp. TCR112. Annual Meeting of the Phytopathological Society of Japan. Tsukuba, Japan (Oral).
  • Marian M., Nishioka T., Koyama H., Suga H., and Shimizu M. (2018). Synergistic effect of Mitsuaria sp. and non-pathogenic Ralstonia sp. on the suppression of tomato bacterial wilt. Annual Meeting of the Japanese Society of Soil Microbiology. Hiroshima, Japan (Poster).
  • Marian M., Nishioka T., Koyama H., Suga H., and Shimizu M. (2018). Synergistic effect of Ralstonia sp. TCR112 and Mitsuaria sp. TWR114 in biocontrol of tomato bacterial wilt. Annual Meeting of the Phytopathological Society of Japan. Kobe, Japan (Oral).
  • Marian M., Koyama H., Suga H., and Shimizu M. (2017). Novel strains of Mitsuaria chitosanitabida and Ralstonia pickettii as biocontrol agents against tomato bacterial wilt. Annual Meeting of the Phytopathological Society of Japan. Iwate, Japan (Oral).
  • Marian M., Khedr A., Nishioka T., Suga H., and Shimizu M. (2015). Isolation of rhizosphere bacteria and evaluation of their biocontrol potential against tomato bacterial wilt. Symposium on the Present and Future Use of Beneficial Microorganisms. Toyama, Japan (Poster).
Altre attività

Patent applications/granted

  • Shimizu M., and Marian M.K.M (2020). Controlling Agent for Bacterial Wilt Disease and Method for Controlling Bacterial Wilt Disease. JP 2020/83556.
  • Satomi S., Ozaki K., Shimizu M., and Marian M.K. (2018). New Strain Belonging to Genus Mitsuaria and Microbial Pesticide Using Said Strain. WO 2018/186307.
  • Satomi S., Ozaki K., Shimizu M., and Marian M.K. (2018). New Strain Belonging to Genus Ralstonia pickettii and Microbial Pesticide Using Said Strain. WO 2018/186312.
  • Satomi S., Ozaki K., Shimizu M., and Marian M.K. (2018). Microbial Pesticide Using A Strain Belonging to Genus Mitsuaria And A Strain Belonging to Genus Ralstonia In Combination. WO 2018/186316.